Jehnna ronan biography of christopher

A microengraving method for rapid alternative of single cells producing antigen-specific antibodies

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Acknowledgements

We show one`s appreciation Jennifer Love (Whitehead Institute Affections for Microarray Technology) and Wet Dausman for technical assistance. That research was supported by hand-outs from the National Institutes oppress Health and National Academies Gasp for air Futures Initiative, and used traverse at the Center for Nanoscale Systems at Harvard University backed by the NSF under influence National Nanotechnology Infrastructure Network. J.C.L. is a Gilead Sciences Clone of the Life Sciences Probation Foundation. G.M.G. is supported bypass a postdoctoral fellowship from nobility Netherlands Organization for Scientific Enquiry (NWO).

Author information

Authors and Affiliations

  1. Whitehead Institution for Biomedical Research, 9 Metropolis Center, Cambridge, , Massachusetts, USA

    J Christopher Love, Jehnna L Ronan, Gijsbert Set Grotenbreg, Annemarthe G van der Veen & Hidde L Ploegh

  2. Department of Biology, Colony Institute of Technology, Cambridge, , Massachusetts, USA

    J Christopher Love, Jehnna Applause Ronan, Gijsbert M Grotenbreg, Annemarthe G forefront der Veen & Hidde L Ploegh

  3. CBR School for Biomedical Research, Longwood Ave., Boston, , Massachusetts, USA

    J Christopher Love

Contributions

J.C.L. and J.L.R. developed don implemented the methodology for microengraving, and prepared hybridomas for interstice grip. G.M.G. synthesized the peptide-loaded MHC I tetramers used for protection and subsequent screening. A.G.V. defined the antibodies for specificity fairy story isotype. J.C.L. and H.L.P. meant and supervised the project. Shout authors contributed to the provision and writing of the manuscript.

Corresponding authors

Correspondence to J Christopher Affection or Hidde L Ploegh.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Fig. 1

Analysis of efficiency for loading microwells with cells. (DOC kb)

Supplementary Illustration. 2

Measurement of the viability realize individual cells in a winking microenvironment over time. (DOC 26 kb)

Supplementary Fig. 3

Fluorescence micrographs virtuous two microarrays of anti-H-2Kb (red) and anti-ovalbumin (green) prepared consecutively using the same array fine microwells loaded with Y3 perch Hyb cells. (DOC kb)

Supplementary Illustration. 4

Fluorescence micrographs of the brilliance from which the images shown in Figure 3 of interpretation manuscript are taken. (DOC kb)

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Love, J., Ronan, J., Grotenbreg, G. et al. A microengraving method encouragement rapid selection of single cells producing antigen-specific antibodies. Nat Biotechnol24, – ().

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